Trinity has an extensive range of in vitro and in vivo inflammation models and assays.
Inflammation is associated with almost every health and medical condition. Many types of cells, mostly leukocytes, may be activated to cause inflammation. This can be manifested through multiple biochemical pathways depending on a range of factors such as:
- The nature of the inflammatory agent
- The site of inflammation
- Age
- Gender
There are circumstances where it is beneficial such as: a response to infections, the early stages of wound healing and sexual function. In contrast, the suppression of inflammation associated with chronic conditions is beneficial and improves the quality of life for conditions such as arthritis, diabetes, psoriasis, cancer and emphysema.
In vitro models
The Trinity team has more than 17 years experience using primary cells to investigate the modulation of the inflammatory response. These primary cells are freshly isolated from blood for the model. Most frequently these are neutrophils, monocytes, macrophages and mast cells.
The general approach is to activate the primary cells with an inflammatory agent, add the preparation being tested and measure the effect on the production of inflammatory mediators. These molecular mediators are numerous. The most frequently studied are growth factors, cytokines such as the numerous interleukins, reactive oxygen species, prostaglandins, thromboxanes and leukotrienes associated with COX and lipoxygenase pathways.
In vivo models
Trinity has a number of in vivo models for investigating medical and health conditions. These include models for, joint conditions, diabetes, metabolic syndrome, wound healing, cancer and gastro-intestinal diseases. These models are detailed in the sites about these conditions.
Models and tests include:
In Vitro | |
Chondrocyte cultures | |
Synoviocyte cultures | |
Neutrophils | |
Primary monocytes | |
Primary macrophages | |
Epithelial cells | |
Keratinocytes | |